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peroxidase vector laboratories sk 4800 vector novared substrate kit  (Vector Laboratories)


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    Vector Laboratories peroxidase vector laboratories sk 4800 vector novared substrate kit
    Peroxidase Vector Laboratories Sk 4800 Vector Novared Substrate Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2019 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peroxidase+vector+laboratories+sk+4800+vector+novared+substrate+kit/VECTOR+NovaRED+Peroxidase+(HRP)+Substrate+Kit/pmc11775210__41467_2025_56565_MOESM1_ESM-102-105-106
    Average 96 stars, based on 2019 article reviews
    peroxidase vector laboratories sk 4800 vector novared substrate kit - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Cholesterol Assay:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Article Title: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm 993-02501 Triglycerides Assay Enzyme Color A FujiFilm 994-02891 Triglycerides Assay Enzyme Color B FujiFilm 990-02991 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 GW3965 Selleck Chemicals 82630 BMS852927 MedChem Express HY-101973 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Anti-FLAG Magnetic Beads MilliporeSigma M8823 3X FLAG Peptide MilliporeSigma F4799 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-FLAG (1/1000) DHSB 12C6c Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Anti-Tim4 (1/1000) Biolegend 130002 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Mouse IgG+IgM Horseradish Peroxidase (1/10,000) Fisher Scientific G21040 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 .. Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Clec4f-Cre-tdTomato Jackson Laboratories 033296 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254

    Glucose Assay:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Article Title: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm 993-02501 Triglycerides Assay Enzyme Color A FujiFilm 994-02891 Triglycerides Assay Enzyme Color B FujiFilm 990-02991 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 GW3965 Selleck Chemicals 82630 BMS852927 MedChem Express HY-101973 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Anti-FLAG Magnetic Beads MilliporeSigma M8823 3X FLAG Peptide MilliporeSigma F4799 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-FLAG (1/1000) DHSB 12C6c Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Anti-Tim4 (1/1000) Biolegend 130002 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Mouse IgG+IgM Horseradish Peroxidase (1/10,000) Fisher Scientific G21040 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 .. Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Clec4f-Cre-tdTomato Jackson Laboratories 033296 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254

    AST Assay:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Article Title: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm 993-02501 Triglycerides Assay Enzyme Color A FujiFilm 994-02891 Triglycerides Assay Enzyme Color B FujiFilm 990-02991 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 GW3965 Selleck Chemicals 82630 BMS852927 MedChem Express HY-101973 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Anti-FLAG Magnetic Beads MilliporeSigma M8823 3X FLAG Peptide MilliporeSigma F4799 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-FLAG (1/1000) DHSB 12C6c Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Anti-Tim4 (1/1000) Biolegend 130002 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Mouse IgG+IgM Horseradish Peroxidase (1/10,000) Fisher Scientific G21040 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 .. Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Clec4f-Cre-tdTomato Jackson Laboratories 033296 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254

    Avidin-Biotin Assay:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Article Title: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm 993-02501 Triglycerides Assay Enzyme Color A FujiFilm 994-02891 Triglycerides Assay Enzyme Color B FujiFilm 990-02991 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 GW3965 Selleck Chemicals 82630 BMS852927 MedChem Express HY-101973 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Anti-FLAG Magnetic Beads MilliporeSigma M8823 3X FLAG Peptide MilliporeSigma F4799 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-FLAG (1/1000) DHSB 12C6c Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Anti-Tim4 (1/1000) Biolegend 130002 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Mouse IgG+IgM Horseradish Peroxidase (1/10,000) Fisher Scientific G21040 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 .. Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Clec4f-Cre-tdTomato Jackson Laboratories 033296 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254

    Blocking Assay:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Article Title: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm 993-02501 Triglycerides Assay Enzyme Color A FujiFilm 994-02891 Triglycerides Assay Enzyme Color B FujiFilm 990-02991 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 GW3965 Selleck Chemicals 82630 BMS852927 MedChem Express HY-101973 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Anti-FLAG Magnetic Beads MilliporeSigma M8823 3X FLAG Peptide MilliporeSigma F4799 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-FLAG (1/1000) DHSB 12C6c Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Anti-Tim4 (1/1000) Biolegend 130002 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Mouse IgG+IgM Horseradish Peroxidase (1/10,000) Fisher Scientific G21040 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 .. Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Clec4f-Cre-tdTomato Jackson Laboratories 033296 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254

    Membrane:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Article Title: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm 993-02501 Triglycerides Assay Enzyme Color A FujiFilm 994-02891 Triglycerides Assay Enzyme Color B FujiFilm 990-02991 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 GW3965 Selleck Chemicals 82630 BMS852927 MedChem Express HY-101973 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Anti-FLAG Magnetic Beads MilliporeSigma M8823 3X FLAG Peptide MilliporeSigma F4799 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-FLAG (1/1000) DHSB 12C6c Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Anti-Tim4 (1/1000) Biolegend 130002 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Mouse IgG+IgM Horseradish Peroxidase (1/10,000) Fisher Scientific G21040 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 .. Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Clec4f-Cre-tdTomato Jackson Laboratories 033296 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254

    Virus:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Bioprocessing:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Software:

    Article Title: A Novel Mutation in LXRα Uncovers a Role for Cholesterol Sensing in Limiting Metabolic Dysfunction-Associated Steatohepatitis (MASH)
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm/Wako 993-02501 Triglycerides Assay Fisher Scientific T7532-120 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 Experimental Models: Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) 6 Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254 Virus and Plasmids Source Identifier AAV-TBG-Cre Addgene 107787-AAV8 AAV-TBG-GFP Addgene 105535-AAV8 pR26 CAG AsiSI/MluI Addgene 74286 pAV-CMV-FLAG-mLXRα-PGK-EGFP VectorBuilder VB200409-1065yuy pAV-CMV-FLAG-mLXRα W441F-PGKEGFP VectorBuilder VB220906-1310evw pAV-CMV-EGFP Vector Builder VB010000-9299haqc Software Source GraphPad Prism 10 GraphPad Software Image-Pro Plus 7.0 Media Cybernetics ImageQuant TL Cytiva Life Sciences 7 ..

    Magnetic Beads:

    Article Title: A mutation in LXRα uncovers a role for cholesterol sensing in limiting metabolic dysfunction-associated steatohepatitis
    Article Snippet: .. Commercial Reagents and Chemicals Source Identifier Total Cholesterol Assay Fisher Scientific TR13421 Free Cholesterol Assay FujiFilm 993-02501 Triglycerides Assay Enzyme Color A FujiFilm 994-02891 Triglycerides Assay Enzyme Color B FujiFilm 990-02991 Glucose Assay Fisher Scientific G7517-120 ALT Assay Fisher Scientific A7526-150 AST Assay Fisher Scientific A7561-150 Bile Acids Assay Fisher Scientific DZ042A-KY1 MASH Diet Teklad TD.96121 24,25-epoxycholesterol Cayman Chemicals 10131 T0901317 Cayman Chemicals 71810 GW3965 Selleck Chemicals 82630 BMS852927 MedChem Express HY-101973 Triazole Fisher Scientific 15596026 BCA Asay Kit Fisher Scientific 23225 Direct Red MilliporeSigma 365548-5G Oil Red O MilliporeSigma O0625-25G BLOXALL Vector Laboratories SP-6000 Avidin,/Biotin Blocking Kit Vector Laboratories SP-2001 Vectastain Elite ABC Reagent, Peroxidase Vector Laboratories SK-4800 Vector NovaRed Substrate kit, Peroxidase Vector Laboratories SK-4800 Hematoxylin QS Vector Laboratories H-3404 Lysing Matrix D Tubes MP Biomedicals 6913500 Quick-RNA Miniprep Kits Zymo Research R1055 Anti-FLAG Magnetic Beads MilliporeSigma M8823 3X FLAG Peptide MilliporeSigma F4799 Immobilon-P membrane MilliporeSigma IPVD00010 Antibody (dilution) Source Identifier Anti-Cd68 (1/1500) Biolegend 137001 Anti-Clec4f (1/1000) R&D Systems 2785-CL Anti-FLAG (1/1000) DHSB 12C6c Anti-GAPDH (1/1000) Cell Signaling 5174S Anti-Ki67 (1/500) Cell Signaling 12202T Anti-LXRα (1/3000) R&D Systems PP-PPZ0412-00 Anti-TAZ (1/1000) R&D Systems 83669S Anti-TBP (1/1000) Cell Signaling 440595 Anti-Tim4 (1/1000) Biolegend 130002 Goat Anti-Mouse IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2192 Goat Anti-Mouse IgG+IgM Horseradish Peroxidase (1/10,000) Fisher Scientific G21040 Goat Anti-Rabbit IgG+IgM Alkaline Phosphatase (1/5000) Fisher Scientific T2191 Goat Anti-Rabbit IgG Biotinylated (1/200) Vector Laboratories BA-1000 Rabbit Anti-Goat IgG Biotinylated (1/200) Vector Laboratories BA-5000 Rabbit Ant-Rat IgG Biotinylated (1/200) Vector Laboratories BA-4001 .. Organisms/Strains Source Identifier Mouse: BgSJLF1/J Jackson Laboratories 100012 Mouse: C57BL/6J Jackson Laboratories 000664 Mouse: Clec4f-Cre-tdTomato Jackson Laboratories 033296 Mouse: Lxrαfl/fl Mangelsdorf Lab (Zhang et al., 2012) Mouse: Lxrα W441F University of Virginia This study Mouse: Rosa26 FLAG-LXRα W441F/ Lxrαfl/fl University of Virginia This study Mouse: Lxrα-/-/Lxrβ-/- Immortalized BMDM Tontonoz Lab (Ito et al., 2015) Mouse: AML12 Cells ATCC CRL-2254



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